Specifications for DIVING-PAM-II

Optoelectronic Unit DIVING-PAM-II/R (RED Version)

Measuring light:Red LED (655 nm ± 5 nm tolerance), modulation frequencies and PAR as described for DIVING-PAM-II/B. Fluorescence at wavelengths greater than 700 nm is measured

Actinic light: Same red LED as for measuring light, maximum PAR of actinic light and saturation pulses as described for DIVING-PAM-II/B

Optoelectronic Unit DIVING-PAM-II/B & DIVING-PAM-II/R

Fluorescence detection: PIN photodiode protected by long-pass and a short-pass filters, 12 bit signal resolution

Pressure and temperature: Piezo-resistive pressure sensor and temperature sensor. Pressure is converted in meters of diving depth, range 0 to -50 m, displayed at 0.1 m intervals. Temperature, range -10 °C to +60 °C, displayed at 0.1 °C intervals. Sensor module with gel protection and antimagnetic stainless steel cap

Data storage: Flash memory, 8 MB, providing memory for more than 27,000 saturation pulse analyses

Display: Backlit 160 x 104 dots (78 x 61 mm) transflective B/W screen

Control elements: 10 infrared reflection switches, pushbutton for saturation pulses, pushbutton to switch device on/off and to lock/unlock reflection switches

WLAN: Wireless LAN Interface, IEEE 802.11 b/g/n (2.4 GHz), Access Point Mode

Design: Plexiglas tube with Plexiglas end plates, one with waterproof fiberoptics port

Mountings: 2 anodized aluminum rods (diameter 15 mm) mounted parallel to the fluorometer body. Anodized aluminum holder for fiber optics. Connections for carrying belt and miniature spectrometer MINI-SPEC

Ports: AUX1 and AUX2, 4-pole, for miniature spectrometer MINI-SPEC; for laboratory use via special adapter: Fiber-Optic Oxygen Meter FireStingO2 or Leaf Clip Holder 2035-B. OUT1 and OUT2, 6-pole, input for trigger signal from Universal Sample Holder DIVING-II-USH; for laboratory use via special adapter: External LED Light Source 2054-L. INPUT, 6-pole, for RS-485 communication and charging of internal battery

Battery: Lead acid battery 8.0 V / 3.5 Ah (28 Wh) providing power for more than 1300 yield measurements

Maximum diving depth: 50 m

Operating temperature: -5 to +45 °C

Dimensions: Diameter 19 cm, length 39 cm

Weight: 3.9 kg

Miniature Spectrometer MINI-SPEC

Design: POM tube, at one side, port for light detection, port for fluorescence excitation by blue (452 nm max) or green (525 nm max) LEDs, and port for white light from a tungsten lamp for reflection measurements; at the opposite side, 4-pole underwater socket

Spectrometer module: Hamamatsu micro-spectrometer, spectral range: 400 to 800 nm, spectral resolution: between 8 and 10 nm. Maximum PAR: 4000 µmol m-2s-1 for illumination having spectral characteristics similar to sunlight

Dimensions: 3.25 cm diameter, 17.5 cm length max

Weight: 135 g

MINI-SPEC Small Parts

Flat Entrance Optics SPEC/P: Design: Hard-anodized aluminum rod of 10 mm diameter and 50 mm length, at one end with lateral light entrance through a 5 mm diameter diffusor and the opposite end inserted in a mounting plate (diameter 33 mm, height 5 mm). Aluminum rod with internal light guide

Fluorescence and Reflection Optics SPEC/R: Design: Spectrometer cap consisting of POM: maximum diameter 35 mm, height 13 mm, weight 16 g. With central 5 mm x 16 mm groove accommodating Perspex light guides for blue and green for fluorescence excitation, and for white light for reflection measurements. With 3 mm diameter central drilled hole as light channel to the detector window of the spectrometer. Including sample cap to fix sample (maximum diameter 40 mm, height 10 mm, weight 8 g). Spectrometer and sample caps padded with foam rubber, both parts have magnets build-in to attract each other and, thus, hold the sample

PAR Calibration Block 000160101439: Design: POM block with drill hole for entrance optics of the Miniature Spectrometer. Oriented at an angle of 60° and 90° relative to the spectrometer port are drill holes for the Fiber Optics DIVING-F

Dimensions: 4.15 cm x 2 cm x 5 cm (L x W x H)

Weight: 40 g

Fiberoptics DIVING-F

Design: Randomized 70 µm glass fibers forming single plastic shielded bundle with stainless steel adapter ends

Dimensions: Active diameter 5.5 mm, outer diameter 8 mm, length 150 cm

Weight: 340 g

Power Supply MINI-PAM-II/N

Input: 100 V to 240 V AC, 50 to 60 Hz

Output: 12 V DC, 5.5 A

Operating temperature: -5 to +45 °C, (non-condensing)

Weight: 350 g including cables

Software WinControl-3

Program: WinControl-3 System Control and Data Acquisition Program (Microsoft Windows 10 and 11) for operation of measuring system via PC, data acquisition and analysis. Not compatible with Windows 10 on ARM

Saturation Pulse Analysis: Measured: Ft, F0, FM, F, F0' (also calculated), FM'. PAR, water temperature and depth (derived from pressure). Calculated: F0' (also measured), FV/FM and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate)

Fitting Routines: Two routines for determination of the cardinal points α, Ik and ETRmax of light curves

Programmed Features: Automatic determination of signal offset for all light intensities and all gain levels. Automatic calibration of internal PAR sensor against PAR from MINI-SPEC sensor

Communication Protocol: USB and IEEE 802.11 b/g/n

Computer Requirements: Processor, 1 GHz. RAM, 512 MB. Screen resolution, 1024 x 600 pixels. Interface, USB 2.0/3.0

PC Interface Box DIVING-PAM-II/I

Housing: Aluminum case with USB-B port, socket for power supply MINI-PAM-II/N, and waterproof 6-pole socket for RS-485 communication

Function: Connects computer and DIVING-PAM-II. RS-485 serial data communication is used between box and DIVING-PAM-II, USB communication is employed between interface box and computer. Recommended maximum cable lengths: 50 m RS-485 cable, 2 m USB cable. Standard USB-A to USB-B cable included

Dimensions: 9.7 cm x 6.3 cm x 3.5 cm (L x W x H)

Weight: 270 g

Operating temperature: -5 °C to + 40 °C

Underwater Cable DIVING-PAM-II/K5

Length: 5 m

Weight: 500 g

Dark Leaf Clip DIVING-LC

Design: Three clips made of white plastic with gasket contact areas and sliding shutter for light-tight closure.

Dimensions: Diameter 3.2 cm, length 8 cm

Weight: 6.5 g

Distance Clip 60° 2010-A

Design: Metal clip with fiber holder and 11 mm diameter sample hole: 5.5 cm x 1.4 cm (L x W)

Fiber holder: 1.2 cm length, mounted 0.7 cm above base, with lateral screw to fix fiber optics. Angle between fiber optics axis and sample plane: 60°. Two spacer rings to vary the distance between fiber end and leaf surface

Surface Holder DIVING-SH

Design: Holder made of grey PVC, equipped with 3 rubber bands and hooks to be attached to creviced surface (e.g. of coral); nylon screws for distance adjustment

Dimensions: 6 cm x 6 cm x 2.5 cm (L x W x H)

Weight: 95 g

Transport Case DIVING-PAM-II/T

Design: Rugged, hard plastic outdoor case with wheels, pull-out handle and custom foam packing

Dimensions: 57 cm x 47 cm x 27 cm (L x W x H)

Weight: 7.7 kg